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Materials and Methods The study was conducted at the Faisalabad abattoir to investigate the prevalence of brucellosis, tuberculosis and paratuberculosis in slaughtered cattle and buffaloes. A total of consecutive animals, including cattle and buffaloes, were included in the study. For this purpose, blood samples from these animals were collected and serum was separated and stored in a freezer till tested by RBPT. The data about each animal, including its tentative age, weight and sex were also recorded. The age of the animal was assessed by an eruption of permanent teeth. To assess the weight of the animal, the girth was measured and then their tentative weights were estimated by using the method as described earlier [20].
The judgment of butchers about the weight was also recorded. The weight of the animals judged by butchers was correlated well with the results of the girth of the animal. The tissues showing lesions suspected for tuberculosis and paratuberculosis were collected. These tissues were fixed in buffered formalin. Tissue sections of 5 micron meters thick were cut and stained with haematoxylin and eosin staining procedure and observed under microscope.
The results revealed that 7.
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The sero-prevalence was low 3. In cattle, The odds of contracting the disease were 3. However, the odds of contracting disease were 1. However, in cattle such significant association was not observed, although it was The odds of contracting disease in females were 1. Tuberculosis Gross lesions of abscessation with creamy yellow coloured exudate and microscopically with the characteristic granuloma formation were considered suspect for tuberculosis. The microscopic lesions seen were having central caseation with occasional calcification, macrophages, giant cells and lymphocytes making a rim around the central necrotic area with fibrous encapsulation.
The prevalence of tuberculosis on this basis was 1. However, in buffaloes, non-significant difference was seen among age groups, although, such lesions were observed in higher percentage of buffaloes of years of age. The odds of contracting disease were 1.
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With respect to sex, not a single male of cattle or buffalo showed such lesions and these lesions were observed only in females Table 2. The odds of contracting disease in female were 1. The results of lesions suspected for tuberculosis in animals of different weight groups revealed non-significant difference Table 3.
However, animals having weight less than kg showed lesions both in cattle and buffaloes. The odds of contracting tuberculosis in animals of less than kg weight were 1.
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Paratuberculosis Gross lesions of corrugated thickening of the last part of the small intestine Ileum and variable parts of the large intestine caecum and part of the colon with thickening of ileocaecal valve were considered as suspected for paratuberculosis. Such lesions under microscope revealed intense granulomatous chronic inflammatory reaction with predominant involvement of macrophages. The results on the prevalence of paratuberculosis on the basis of suspected lesions revealed 4.
However, the prevalence was relatively higher in buffaloes of more than 10 years 6. Similarly, non-significant difference was observed in each cattle and buffaloes with respect to sex of the animal Table 2. The odds of contracting disease were 3.
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The prevalence of paratuberculosis did not vary between animals of different weight groups, each in cattle and buffaloes Table 3. The odds of contracting disease in animals of less than kg weight were 1. Discussion Brucellosis has been reported from all over the world. The results of the present study also suggest that both cattle and buffalo are equally susceptible to brucellosis. These invitation-only parties are a rare opportunity for gay men to be open about their sexuality.
Pakistani society is fiercely patriarchal. Pakistanis are expected to marry a member of the opposite sex, and the vast majority do. Sex between men occurs in some very public places - including, surprisingly, Karachi's busiest shrine. Families go to the Abdullah Shah-Ghazi shrine to honour the holy man buried there and to ask for God's blessings, but it is also Karachi's biggest cruising ground.
Every Thursday evening, as the sun sets, men from across the city gather there.